TNF-alpha Stimulates Growth of Human Bone Marrow Stromal Cells
Author Information
Author(s): F. Rougier, E. Cornu, N. Gachard, V. Praloran, Y. Denizot
Primary Institution: Laboratoire d'Hematologie Experimentale, Faculte de Medecine, Limoges, France
Hypothesis
Does TNF-alpha stimulate the growth of human bone marrow stromal cells?
Conclusion
TNF-alpha is a potent mitogen for human bone marrow stromal cells, while other cytokines tested had no effect.
Supporting Evidence
- TNF-alpha increased DNA synthesis by 230% in growth synchronized cells.
- TNF-alpha was effective in stimulating cell growth in 15 out of 15 different donors.
- Other tested cytokines had no significant effect on DNA synthesis.
Takeaway
This study found that a substance called TNF-alpha helps certain cells in our bones grow, while other similar substances do not.
Methodology
Human bone marrow stromal cells were cultured and stimulated with TNF-alpha and other cytokines, with DNA synthesis measured using [3H]-thymidine incorporation.
Participant Demographics
Cells were obtained from 25 different donors.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
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