Circulating C3 and Autoantibody-Mediated Arthritis in Mice
Author Information
Author(s): Paul A Monach, Admar Verschoor, Jonathan P Jacobs, Michael C Carroll, Amy J Wagers, Christophe Benoist, Diane Mathis
Primary Institution: Brigham and Women's Hospital and Harvard Medical School
Hypothesis
Is C3 synthesized within the synovium important for promoting inflammation in rheumatoid arthritis?
Conclusion
Circulating C3 is necessary and sufficient for the induction of arthritis in a mouse model.
Supporting Evidence
- Circulating C3 was found to be necessary for arthritis induction in the K/BxN mouse model.
- Bone marrow chimeras showed that C3 synthesis by radioresistant cells was sufficient for arthritis susceptibility.
- Parabionts with C3 only in circulation developed arthritis, confirming the role of circulating C3.
Takeaway
The study found that a protein called C3 in the blood is really important for causing arthritis in mice, even if it doesn't come from the joints themselves.
Methodology
The study used bone marrow chimeras and parabiotic mice to assess the role of C3 in arthritis induction.
Limitations
The study was conducted in mice, which may not fully replicate human rheumatoid arthritis.
Participant Demographics
Mice used in the study included C3−/− and wild-type strains.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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