Spectrophotometric determination of creatinine by monosegmented continuous-flow analysis
1992

Determining Creatinine in Urine Using Continuous-Flow Analysis

Sample size: 10 publication Evidence: moderate

Author Information

Author(s): Lourival C. de Faria, Celio Pasquini

Primary Institution: Instituto de Quimica, Universidade Estadual de Campinas

Hypothesis

The monosegmented continuous-flow system (MCFS) can effectively determine creatinine levels in urine.

Conclusion

The MCFS method for creatinine determination is comparable in accuracy to standard methods while allowing for higher sample throughput.

Supporting Evidence

  • The MCFS allows for 130 determinations per hour with a relative standard deviation of less than 1.5%.
  • The results from the MCFS method agreed with those obtained by standard manual and automated methods.
  • The correlation coefficient between the MCFS and Cobas-Mira results was 0.9980.

Takeaway

This study shows a new way to test for creatinine in urine that is faster and just as accurate as older methods.

Methodology

The study used a monosegmented continuous-flow system to analyze urine samples for creatinine concentration.

Limitations

The study does not address potential interferences from other substances in urine.

Participant Demographics

Urine samples were supplied by the State University of Campinas's hospital.

Statistical Information

Statistical Significance

p<0.05

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