Transcriptional factors associated with epithelial-mesenchymal transition in choroidal neovascularization
2011
Transcriptional Factors in Eye Disease
Sample size: 12
publication
Evidence: moderate
Author Information
Author(s): Hirasawa Manabu, Noda Kousuke, Noda Setsuko, Suzuki Misa, Ozawa Yoko, Shinoda Kei, Inoue Makoto, Ogawa Yoko, Tsubota Kazuo, Ishida Susumu
Primary Institution: Keio University School of Medicine
Hypothesis
EMT of RPE cells is involved in the fibrotic scar formation in AMD.
Conclusion
Snail is a major transcriptional factor for EMT changes of RPE cells in human CNV.
Supporting Evidence
- 91.6% of CNV tissues showed Snail localized in RPE cell nuclei.
- Snail was co-localized with α-smooth muscle antigen in RPE cells.
- TGF-β and VEGF stimulation increased Snail expression in cultured RPE cells.
Takeaway
This study found that a protein called Snail helps eye cells change shape and contribute to scarring in a common eye disease.
Methodology
The study used tissue samples from patients and cultured RPE cells to analyze the expression of EMT-related transcriptional factors.
Participant Demographics
12 patients with AMD, 8 males and 4 females, mean age 67.3 years.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
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