Differences in Long Non-Coding RNA Hotair Between Mice and Humans
Author Information
Author(s): Patrick Schorderet, Denis Duboule
Primary Institution: Federal Institute of Technology (EPFL), Lausanne, Switzerland
Hypothesis
Is the regulatory mechanism of Hotair conserved between mice and humans during development?
Conclusion
The mouse Hotair lincRNA has evolved differently from its human counterpart, showing little regulatory effect on Hoxd genes in mice.
Supporting Evidence
- The mouse Hotair is poorly conserved in sequence compared to human HOTAIR.
- Deletion of mHotair in mice has little effect on the expression of Hoxd genes.
- The function of mHotair in mice is distinct from that reported for human cells.
Takeaway
This study found that the Hotair RNA in mice works differently than in humans, and it doesn't seem to help control certain genes in mice like it does in humans.
Methodology
The study involved sequence alignment, in situ hybridization, and transcriptome analysis in mouse embryos.
Limitations
The study may not capture all potential effects of mHotair due to the complexity of gene regulation and the specific developmental stages examined.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website