Measuring Lysozyme Activity in Hen Egg White Using Fluorescence Polarization
Author Information
Author(s): Liliya I. Mukhametova, Dmitry O. Zherdev, Sergei A. Eremin, Pavel A. Levashov, Hans-Christian Siebert, Yury E. Tsvetkov, Olga N. Yudina, Vadim B. Krylov, Nikolay E. Nifantiev
Primary Institution: M.V. Lomonosov Moscow State University
Hypothesis
Can synthetic chitooligosaccharides be effectively used to measure active lysozyme in hen egg white?
Conclusion
The fluorescence polarization assay (FPA) provides a reliable method for measuring lysozyme activity in hen egg white, correlating well with traditional methods.
Supporting Evidence
- The FPA method showed a strong correlation with traditional turbidimetry results.
- Lysozyme levels measured by FPA were consistent across different egg samples.
- FPA allows for rapid analysis without the need for bacterial cells.
Takeaway
This study shows a new way to check how much lysozyme is in egg whites using a special test that is faster and easier than older methods.
Methodology
The study used fluorescence polarization assay (FPA) with synthetic chitooligosaccharides to measure lysozyme activity and compared results with traditional turbidimetry.
Potential Biases
Potential for false positives due to the presence of inactivated lysozyme that can bind to tracers.
Limitations
The FPA may be affected by changes in solution viscosity or laboratory temperature.
Participant Demographics
Hen egg whites from various producers in Moscow.
Statistical Information
P-Value
p<0.05
Confidence Interval
95%
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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