Using a Fluorescent Dye to Mark Blood Vessels in Tumors
Author Information
Author(s): M.J. Trotter, D.J. Chaplin, P.L. Olive
Primary Institution: Medical Biophysics Unit, BC Cancer Research Centre
Hypothesis
Can the carbocyanine dye DiOC7(3) effectively mark functional vasculature in murine tumors?
Conclusion
DiOC7(3) can be used to identify functional vasculature in experimental tumors, especially when used alongside Hoechst 33342.
Supporting Evidence
- DiOC7(3) was well tolerated by mice at a dose of 1 mg/kg.
- The dye has a distribution half-life in blood of 180 seconds.
- DiOC7(3) caused a 75% reduction in tumor blood flow as measured by laser Doppler techniques.
- Fluorescent staining patterns were similar to those obtained using Hoechst 33342.
- DiOC7(3) can be used in double staining protocols to identify regions of transient perfusion.
Takeaway
Researchers used a special dye to see blood vessels in tumors, helping them understand how blood flows in cancer.
Methodology
Mice were injected with DiOC7(3) and Hoechst 33342, and their tumors were analyzed for blood vessel staining and blood flow.
Limitations
The dye causes a significant reduction in tumor blood flow, which may affect results.
Participant Demographics
C3H/He mice were used in the study.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
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