Study of Radiation Effects on Stem Cells from Human Teeth
Author Information
Author(s): Abe Shigehiro, Hamada Keiichi, Yamaguchi Satoshi, Amagasa Teruo, Miura Masahiko
Primary Institution: Tokyo Medical and Dental University
Hypothesis
The study aims to characterize the radiobiological properties of stem/progenitor cells derived from apical papilla-derived cells (APDCs) compared to bulk APDCs.
Conclusion
Stem/progenitor cells derived from APDCs exhibit a radioresistant phenotype, but their ability to form hard tissue in vivo is significantly reduced after irradiation.
Supporting Evidence
- PSFCs exhibited a radioresistant phenotype compared to bulk APDCs.
- Irradiation induced a significant increase in a senescence-like phenotype in both cell types.
- Neither type of cells exhibited significant apoptotic changes after 8 Gy of irradiation.
- Hard tissue formation ability in vivo was significantly decreased in PSFCs after 4 Gy of irradiation.
Takeaway
The study found that certain stem cells from teeth can survive radiation better than other cells, but they struggle to create new hard tissue after being exposed to radiation.
Methodology
APDCs were isolated from human third molars and subjected to γ-irradiation, followed by radiosensitivity and hard tissue-forming assays.
Limitations
The study does not specify the exact number of samples used or the long-term effects of radiation on these cells.
Participant Demographics
Human donors of third molars with immature apices.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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