How c-Myc and Sp1 Regulate BRD7 Gene Expression
Author Information
Author(s): Liu Huaying, Zhou Ming, Luo Xiaomin, Zhang Liming, Niu Zhaoxia, Peng Cong, Ma Jian, Peng Shuping, Zhou Houde, Xiang Bo, Li Xiayu, Li Shufang, He Jiajin, Li Xiaoling, Li Guiyuan
Primary Institution: Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Changsha, Hunan, People's Republic of China
Hypothesis
The study investigates the transcriptional regulation of the BRD7 gene by c-Myc and Sp1.
Conclusion
c-Myc negatively regulates BRD7 gene expression, while Sp1 has a slight positive regulatory effect.
Supporting Evidence
- The minimal promoter of BRD7 was defined in a 55-bp region.
- c-Myc was found to negatively regulate BRD7 promoter activity.
- Sp1 was shown to bind to the BRD7 promoter and slightly increase its activity.
- Knockdown of c-Myc led to increased BRD7 promoter activity and mRNA expression.
Takeaway
This study shows that a protein called c-Myc can stop another protein called BRD7 from working properly, while another protein, Sp1, helps it a little bit.
Methodology
The study used luciferase assays, RT-PCR, western blot assays, EMSA, and ChIP to analyze the regulation of BRD7 gene expression.
Digital Object Identifier (DOI)
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