Platelet-derived exosomes from septic shock patients induce myocardial dysfunction
Author Information
Author(s): Azevedo Luciano Cesar Pontes, Janiszewski Mariano, Pontieri Vera, Pedro Marcelo de Almeida, Bassi Estevão, Tucci Paulo José Ferreira, Laudindo Francisco Rafael Martins
Primary Institution: University of São Paulo School of Medicine
Hypothesis
Circulating platelet-derived exosomes could contribute to inotropic dysfunction of sepsis.
Conclusion
Exosomes from septic patients induce myocardial dysfunction in isolated heart and papillary muscle preparations.
Supporting Evidence
- Exosomes from septic patients significantly decreased positive and negative derivatives of left ventricular pressure in isolated rabbit hearts.
- Exosomes from healthy individuals decreased these variables non-significantly.
- The negative inotropic effect of septic exosomes was fully reversible upon withdrawal of exosomes.
- Nitric oxide production from exosomes derived from septic shock patients was demonstrated by fluorescence.
- There was an increase in myocardial nitrate content after exposure to septic exosomes.
Takeaway
Exosomes from the blood of patients with septic shock can make the heart work less effectively, which can be reversed if the exosomes are removed.
Methodology
Blood samples were collected from 55 patients with septic shock and 12 healthy volunteers for exosome separation and their effects on isolated heart and papillary muscle preparations were investigated.
Limitations
The isolated heart or papillary muscle preparations may not fully represent the effects of exosomes on cardiomyocytes alone.
Participant Demographics
55 patients with septic shock and 12 healthy volunteers.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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