Chromatin Accessibility and Gene Expression in Maize Leaf Development
Author Information
Author(s): Wang Yiduo, Wang Shuai, Wu Yufeng, Cheng Jiawen, Wang Haiyan, Wang Mingcheng
Primary Institution: Nanjing Agricultural University
Hypothesis
The study aimed to investigate the dynamics of chromatin accessibility and its influence on genome-wide gene expression during different stages of maize leaf development.
Conclusion
Chromatin accessibility plays a crucial role in regulating the spatial and temporal expression of key genes during maize leaf development.
Supporting Evidence
- A total of 46,808, 38,242, and 41,084 accessible chromatin regions were identified at the BBCH_11, BBCH_13, and BBCH_17 stages, respectively.
- Integrated analyses revealed that both the number and intensity of accessible chromatin regions significantly influence gene expression levels.
- Motif analysis identified key transcription factors associated with leaf development.
Takeaway
This study shows that how open or closed the DNA is can change how genes are turned on or off as maize leaves grow.
Methodology
Maize leaves were collected at different developmental stages, and chromatin accessibility was assessed using ATAC-seq, while RNA-seq was performed to profile gene expression.
Statistical Information
P-Value
0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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