Integrated Analysis of Transcriptome and Metabolome in the Brain After Cold Stress of Red Tilapia During Overwintering
2024

Understanding Cold Stress in Red Tilapia

Sample size: 80 publication 10 minutes Evidence: moderate

Author Information

Author(s): Zhu Chenxi, Yang Haoran, Zhu Wenbin, Jiang Qichen, Dong Zaijie, Wang Lanmei

Primary Institution: Freshwater Fisheries Research Centre of Chinese Academy of Fishery Sciences

Hypothesis

What are the regulatory mechanisms of cold stress in red tilapia during overwintering?

Conclusion

Cold stress may promote apoptosis in red tilapia through the FoxO signaling pathway and affect amino acid metabolism via ABC transporters.

Supporting Evidence

  • Cold stress led to significant changes in serum physiological parameters of red tilapia.
  • Transcriptome sequencing and metabolome analysis revealed key pathways affected by cold stress.
  • The FoxO signaling pathway was identified as crucial for regulating apoptosis under cold stress.
  • ABC transporters were found to play a role in amino acid metabolism in response to cold stress.

Takeaway

When red tilapia get too cold, it can make them sick and even die. This study helps us understand how to make them stronger against the cold.

Methodology

The study involved dividing red tilapia into cold stress and control groups, measuring physiological parameters, and conducting transcriptome and metabolome analyses.

Participant Demographics

Red tilapia (Oreochromis spp.) with an initial weight of 72.71 ± 1.32 g.

Statistical Information

P-Value

p<0.05

Confidence Interval

95%

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.3390/ijms252413372

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