The Roots of Diversity: Below Ground Species Richness and Rooting Distributions in a Tropical Forest Revealed by DNA Barcodes and Inverse Modeling
2011

Understanding Below Ground Species Richness in a Tropical Forest

Sample size: 12 publication Evidence: moderate

Author Information

Author(s): Jones F. Andrew, Erickson David L., Bernal Moises A., Bermingham Eldredge, Kress W. John, Herre Edward Allen, Muller-Landau Helene C., Turner Benjamin L.

Primary Institution: Smithsonian Tropical Research Institute

Hypothesis

Can DNA barcoding and inverse modeling reveal patterns of root species richness and distribution in a tropical forest?

Conclusion

DNA barcoding techniques can effectively identify root species and model their distributions, revealing high species richness and potential for underground interactions.

Supporting Evidence

  • 33 species of roots were identified from 117 fragments sequenced.
  • The study recovered approximately 14% of the measured woody plant richness in the focal hectare.
  • Inverse modeling indicated a high potential for underground interactions among plants.
  • Sampling required significantly larger areas to match above-ground species richness.

Takeaway

Scientists used special DNA techniques to find out how many different types of plant roots are in the ground in a tropical forest, and they discovered a lot more than they expected.

Methodology

The study involved sampling soil cores from a tropical forest, extracting DNA from root fragments, and using DNA barcoding to identify species.

Limitations

The study's sampling was limited in both spatial extent and the number of DNA sequences recovered, potentially underestimating true root diversity.

Digital Object Identifier (DOI)

10.1371/journal.pone.0024506

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