Pitting of Malaria Parasites and Spherocyte Formation
Author Information
Author(s): Anyona Samuel B, Schrier Stanley L, Gichuki Charity W, Waitumbi John N
Primary Institution: Kenyatta University, Nairobi, Kenya
Hypothesis
The study investigates whether spherocytes reflect the pitting of malaria parasites from infected red blood cells.
Conclusion
Pitting of malaria parasites may produce spherocytes in children living in malaria holoendemic areas.
Supporting Evidence
- High prevalence of spherocytes was detected in blood smears of children with malaria.
- Co-incubation of infected red blood cells with macrophages led to significant pitting.
- RBCs with pitted parasites showed morphological abnormalities, including spherocyte formation.
Takeaway
The study found that in children with malaria, some red blood cells lose parts of their membrane while getting rid of parasites, which creates a new type of cell called spherocytes.
Methodology
An in vitro model was developed to study pitting and spherocyte formation using Plasmodium falciparum infected red blood cells co-incubated with human macrophages, and blood specimens from patients were analyzed.
Limitations
The study may not account for all host factors influencing pitting and spherocyte formation.
Participant Demographics
Children enrolled in a case control study in the malaria holoendemic Lake Victoria basin.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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