Accelerating Potato Disease Resistance Discovery
Author Information
Author(s): Vleeshouwers Vivianne G. A. A., Rietman Hendrik, Krenek Pavel, Champouret Nicolas, Young Carolyn, Oh Sang-Keun, Wang Miqia, Bouwmeester Klaas, Vosman Ben, Visser Richard G. F., Jacobsen Evert, Govers Francine, Kamoun Sophien, Van der Vossen Edwin A. G.
Primary Institution: Wageningen UR Plant Breeding, Wageningen, The Netherlands
Hypothesis
P. infestans RXLR effectors are candidate Avr genes that can be functionally profiled on Solanum to detect cognate R genes.
Conclusion
Effector genomics enables the rapid discovery and functional profiling of late blight resistance genes in potatoes.
Supporting Evidence
- 54 RXLR effectors were profiled for their ability to activate innate immunity in wild Solanum species.
- Specific RXLR effectors triggered hypersensitivity in resistant but not in susceptible potato genotypes.
- Genetic studies showed cosegregation of resistance to P. infestans and response to identified effectors.
Takeaway
Scientists found a way to quickly identify genes that help potatoes resist a harmful disease by studying proteins from the disease-causing organism.
Methodology
The study involved expressing 54 candidate RXLR effectors in late blight resistant Solanum plants to identify effector responses.
Limitations
The PVX agroinfection method may lead to false positives due to high expression levels of single effectors.
Participant Demographics
The study involved various wild Solanum species and potato cultivars.
Digital Object Identifier (DOI)
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