Claudin 4 and Ovarian Cancer
Author Information
Author(s): Kristin L. M. Boylan, Benjamin Misemer, Melissa S. DeRycke, John D. Andersen, Katherine M. Harrington, Steve E. Kalloger, C. Blake Gilks, Stefan E. Pambuccian, Amy P. N. Skubitz
Primary Institution: University of Minnesota
Hypothesis
Is the expression of claudin 4 associated with outcomes in ovarian cancer patients and involved in tumor progression?
Conclusion
Claudin 4 is overexpressed in ovarian cancer tissues but does not correlate with disease-specific survival.
Supporting Evidence
- Claudin 4 was expressed in nearly 70% of the ovarian cancer tissues examined.
- Claudin 4 expression was differentially observed across ovarian cancer subtypes.
- Claudin 4 was found in multicellular spheroids obtained from patients’ ascites.
- NIH:OVCAR5 cells treated with shRNA against claudin 4 required a longer time to form compact spheroids.
Takeaway
Claudin 4 is a protein that helps cells stick together, and it is found in many ovarian cancer tissues, but it doesn't help predict how long patients will live.
Methodology
The study examined claudin 4 expression in ovarian cancer tissues and cell lines using RT-PCR, qRT-PCR, Western immunoblotting, and immunohistochemistry on tissue microarrays.
Limitations
No association was found between claudin 4 expression and disease-specific survival in any subtype.
Participant Demographics
The study included 500 cases of epithelial ovarian cancer of different subtypes.
Statistical Information
P-Value
0.0026
Statistical Significance
p = 0.0026
Digital Object Identifier (DOI)
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