Thrombin Generation by Activated Factor VII on Platelets
Author Information
Author(s): Altman Raul, Scazziota Alejandra Silvia, Herrera Maria de Lourdes, Gonzalez Claudio
Primary Institution: Centro de Trombosis de Buenos Aires, Buenos Aires, Argentina
Hypothesis
Does activated Factor VII in combination with platelet agonists increase thrombin generation in vitro?
Conclusion
Platelets activated by AA, ADP, Col, or rFVIIa triggered thrombin generation, which was enhanced by combining rFVIIa with other agonists.
Supporting Evidence
- Activated Factor VII combined with platelet agonists significantly decreased lag time and time to peak thrombin generation.
- Thrombin generation parameters were significantly increased in activated platelet rich plasma compared to non-activated plasma.
- The study used a calibrated automated thrombography technique to measure thrombin generation.
Takeaway
This study shows that certain substances can make blood cells called platelets work better to help stop bleeding by creating a substance called thrombin.
Methodology
Thrombin generation was measured using the calibrated automated thrombography technique in platelet rich plasma from healthy volunteers after activation with various agonists.
Potential Biases
Potential bias from platelet activation during blood collection and preparation.
Limitations
The study's results may not directly apply to patients, and the physiological concentrations of agonists in vivo are unknown.
Participant Demographics
29 healthy volunteers, 20 women and 9 men, ages 22 to 72.
Statistical Information
P-Value
p = 0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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