Gene Expression Profiles of Human Embryonic Stem Cells
Author Information
Author(s): Liu Ying, Shin Soojung, Zeng Xianmin, Zhan Ming, Gonzalez Rodolfo, Mueller Franz-Josef, Schwartz Catherine M, Xue Haipeng, Li Huai, Baker Shawn C, Chudin Eugene, Barker David L, McDaniel Timothy K, Oeser Steffen, Loring Jeanne F, Mattson Mark P, Rao Mahendra S
Primary Institution: National Institute on Aging Intramural Research Program, National Institutes of Health
Hypothesis
Can gene expression profiles of human embryonic stem cell lines and their derivatives be compared to monitor feeder contaminations?
Conclusion
The Illumina bead array platform is a reliable method for developing global profiles of cells and identifying similarities and differences in a large number of samples.
Supporting Evidence
- The study analyzed gene expression in seven hESC lines and human fibroblast feeder cells.
- Hierarchical clustering showed that blinded duplicates were correctly identified as the closest related samples.
- hESC lines clustered together irrespective of the laboratory in which they were maintained.
- The embryonal carcinoma line NTera2 is a useful model for evaluating characteristics of hESCs.
Takeaway
Scientists studied different types of stem cells to see how they grow and change, using a special tool to look at their genes.
Methodology
Gene expression was analyzed using Illumina bead arrays containing probes for 24,131 transcript probes across 48 samples.
Limitations
The study may not account for all variations in gene expression due to different culture conditions.
Participant Demographics
Included various human embryonic stem cell lines and fibroblast feeder cells.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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