Removal of spermatozoa with externalized phosphatidylserine from sperm preparation in human assisted medical procreation: effects on viability, motility and mitochondrial membrane potential
2009

Removing Damaged Sperm to Improve Fertility

Sample size: 44 publication 10 minutes Evidence: moderate

Author Information

Author(s): de Vantéry Arrighi Corinne, Lucas Hervé, Chardonnens Didier, de Agostini Ariane

Primary Institution: Geneva University Hospitals and University of Geneva

Hypothesis

Can the removal of spermatozoa with externalized phosphatidylserine enhance sperm quality for assisted medical procreation?

Conclusion

Removing sperm with externalized phosphatidylserine improves sperm viability, motility, and mitochondrial integrity.

Supporting Evidence

  • 14% of spermatozoa were found to have externalized phosphatidylserine.
  • MACS combined with DGC reduced EPS spermatozoa by 70%.
  • Sperm survival increased by 50% at 24 hours after treatment.

Takeaway

This study shows that getting rid of unhealthy sperm can help make the remaining sperm better for having babies.

Methodology

Sperm was prepared using density gradient centrifugation and magnetic-activated cell sorting to remove unhealthy sperm.

Potential Biases

Potential bias in patient selection and sperm quality assessment.

Limitations

The study focused on a specific population of infertile patients, which may limit generalizability.

Participant Demographics

Infertile men attending a reproductive medicine unit.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1477-7827-7-1

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