Pharmacokinetic studies in mice with ICI D0490, a novel recombinant ricin A-chain immunotoxin
1993

Pharmacokinetics of ICI D0490 in Mice

Sample size: 6 publication Evidence: moderate

Author Information

Author(s): J.A. Calvete, D.R. Newell, C.J. Charlton, A.F. Wright

Primary Institution: The Cancer Research Unit, University of Newcastle upon Tyne

Hypothesis

The pharmacokinetic advantages of using a hindered disulphide linker and recombinant ricin A-chain in ICI D0490 have been achieved.

Conclusion

ICI D0490 has excellent in vivo stability and persistence, making it a promising candidate for clinical evaluation in colorectal cancer treatment.

Supporting Evidence

  • ICI D0490 showed a long elimination phase half-life of 33 hours.
  • Plasma levels of ICI D0490 were sustained at over 20 µg/ml for 8 hours after administration.
  • Clearance of ICI D0490 from plasma was extremely slow at 34 µl/min/kg.

Takeaway

Researchers studied a new cancer treatment in mice and found it stays in the body for a long time, which could help fight cancer better.

Methodology

The pharmacokinetics of ICI D0490 were studied in mice using intravenous administration and measuring plasma concentrations over time with ELISA.

Limitations

The study was conducted in non-tumour bearing mice, which may not fully represent the pharmacokinetics in cancer patients.

Participant Demographics

Six 8-week-old female Balb/c mice were used.

Statistical Information

Confidence Interval

95% confidence limits for LDIO values were calculated.

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