CREB Expression and Alzheimer's Disease
Author Information
Author(s): Pugazhenthi Subbiah, Wang Maorong, Pham Serena, Sze Chun-I, Eckman Christopher B
Primary Institution: University of Colorado Denver
Hypothesis
Chronic downregulation of CREB-mediated transcription contributes to the progression of Alzheimer's disease.
Conclusion
The study suggests that decreased CREB expression in Alzheimer's brain may exacerbate disease progression.
Supporting Evidence
- CREB mRNA levels decreased by 33% in Tg2576 mouse hippocampal neurons.
- Immunohistochemical analysis showed a 40% decrease in CREB protein levels in Tg2576 mice.
- An inverse correlation was found between Aβ levels and CREB content in Alzheimer's post-mortem samples.
- Overexpression of CREB protected against Aβ-induced neuronal apoptosis.
Takeaway
This study found that a protein called CREB, which helps brain cells survive, is lower in Alzheimer's disease, possibly making the disease worse.
Methodology
The study used laser capture microdissection, Western blot analysis, and immunohistochemical staining to assess CREB levels in various models of Alzheimer's disease.
Limitations
The study primarily focuses on specific models and may not fully represent all aspects of Alzheimer's disease.
Participant Demographics
The study included post-mortem samples from individuals with Alzheimer's disease and age-matched controls.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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