SIV Antigen Immunization and T Cell Responses in Rhesus Macaques
Author Information
Author(s): Hu Haitao, Gama Lucio, Aye Pyone P, Clements Janice E, Barry Peter A, Lackner Andrew A, Weissman Drew
Primary Institution: University of Pennsylvania
Hypothesis
Does SIV antigen stimulation in ART-treated rhesus macaques affect T cell responses and viral replication?
Conclusion
SIV antigen immunization induces transient T cell responses and activates viral replication in draining lymph nodes.
Supporting Evidence
- Both SIV and RhCMV immunizations stimulated transient T cell responses.
- RhCMV-specific responses were sustained for 50 days, while SIV-specific responses were transient.
- SIV antigen stimulation led to increased viral replication in draining lymph nodes.
Takeaway
When monkeys were given a vaccine for SIV, their immune system reacted for a short time, but it also made the virus replicate more in certain areas.
Methodology
Rhesus macaques were immunized with SIV gag and RhCMV pp65 plasmids, and immune responses were analyzed in draining lymph nodes and peripheral blood.
Potential Biases
Potential bias due to the small number of animals and the specific immunization regimen used.
Limitations
The study involved a small sample size and focused on short-term responses.
Participant Demographics
Rhesus macaques, Mamu A*01 positive, RhCMV seropositive.
Statistical Information
P-Value
0.0007
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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