Human Herpesvirus-6 Induces MVB Formation, and Virus Egress Occurs by an Exosomal Release Pathway
2008
Human Herpesvirus-6 Induces MVB Formation and Virus Release via Exosomal Pathway
publication
Evidence: high
Author Information
Author(s): Mori Yasuko, Koike Masato, Moriishi Eiko, Kawabata Akiko, Tang Huamin, Oyaizu Hiroko, Uchiyama Yasuo, Yamanishi Koichi
Primary Institution: National Institute of Biomedical Innovation
Hypothesis
How does human herpesvirus-6 (HHV-6) assemble and release from T cells?
Conclusion
HHV-6 is released from infected T cells through multivesicular bodies (MVBs) via an exosomal pathway.
Supporting Evidence
- Electron microscopy showed that HHV-6-infected cells were larger than uninfected cells.
- Mature virions were found in MVBs that fused with the plasma membrane.
- Immunoelectron microscopy demonstrated that MVBs contained CD63 and HHV-6 envelope glycoproteins.
- HHV-6 infection induced a significant increase in the volume density of MVB-like structures.
Takeaway
The study shows that a virus called HHV-6 uses tiny bubbles inside cells to help it escape and spread to other cells.
Methodology
Electron microscopy and immunoelectron microscopy were used to observe the intracellular pathways of HHV-6 assembly and release.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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