B Cells Transmit HIV to T Cells
Author Information
Author(s): Giovanna Rappocciolo, Paolo Piazza, Craig L. Fuller, Todd A. Reinhart, Simon C. Watkins, David T. Rowe, Mariel Jais, Phalguni Gupta, Charles R. Rinaldo Jr.
Primary Institution: University of Pittsburgh
Hypothesis
DC-SIGN on B cells is required for the transmission of HIV-1 to T lymphocytes.
Conclusion
Activated B cells can transmit HIV-1 to T cells through the DC-SIGN pathway, which may play a significant role in HIV-1 pathogenesis.
Supporting Evidence
- B cells expressing DC-SIGN can capture and internalize HIV-1.
- Activated B cells can enhance the transmission of HIV-1 to T cells.
- Blocking DC-SIGN on B cells inhibits HIV-1 transmission to T cells.
Takeaway
B cells can help spread HIV to T cells, which is bad because T cells are important for fighting infections.
Methodology
The study involved flow cytometry to analyze DC-SIGN expression on B cells and co-culture experiments to assess HIV-1 transmission to T cells.
Limitations
The study primarily focused on in vitro experiments, which may not fully replicate in vivo conditions.
Participant Demographics
Healthy, HIV-1 seronegative donors and HIV-1 seropositive subjects.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.0001
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website