Modulation of oxygen consumption rate and vascular endothelial growth factor mRNA expression in human malignant glioma cells by hypoxia
1999
How Glioma Cells Respond to Low Oxygen
Sample size: 5
publication
Author Information
Author(s): Allalunis-Turner M J, Franko A J, Parliament M B
Primary Institution: University of Alberta
Hypothesis
Do human malignant glioma cells vary in their response to hypoxic stress over different oxygen concentrations?
Conclusion
Different human malignant glioma cell lines respond differently to low oxygen levels, which may affect the effectiveness of hypoxia-targeting therapies.
Supporting Evidence
- Decreased oxygen availability resulted in decreased rates of oxygen utilization in glioma cells.
- In two cell lines, modest hypoxia increased vascular endothelial growth factor mRNA levels compared to aerobic controls.
- In two additional cell lines, vascular endothelial growth factor mRNA was constitutively expressed under aerobic conditions and was not further increased by hypoxia.
Takeaway
Some brain cancer cells react differently when there's less oxygen, which could change how well treatments work.
Methodology
Northern blot analysis was used to study the induction of vascular endothelial growth factor mRNA in response to hypoxia in five glioma cell lines.
Digital Object Identifier (DOI)
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