Study of Myxobacter Sp. AL-1 Cellulases
Author Information
Author(s): Ramírez-Ramírez Norma, Romero-García Eliel R., Calderón Vianney C., Avitia Claudia I., Téllez-Valencia Alfredo, Pedraza-Reyes Mario
Primary Institution: Instituto de Investigación en Biología Experimental (IIBE), Facultad de Química, Universidad de Guanajuato
Hypothesis
Myxobacter Sp. AL-1 possesses the enzymatic machinery to degrade cellulose through the synergistic action of Cel9 and Cel48 cellulases.
Conclusion
The study demonstrates that the cel9-cel48 cluster in Myxobacter Sp. AL-1 encodes functional enzymes that work together to degrade cellulose.
Supporting Evidence
- The cel9-cel48 cluster encodes functional endo- and exo-acting cellulases.
- Cel48 showed a synergism of 1.68 times with Cel9 during cellulose degradation.
- Both proteins were synthesized and secreted by Myxobacter Sp. AL-1.
Takeaway
Myxobacter Sp. AL-1 has special proteins that help it break down plant material, and these proteins work better together than alone.
Methodology
The study involved expressing and purifying Cel9 and Cel48 proteins in E. coli and Bacillus subtilis, followed by enzyme activity assays.
Limitations
The production of Cel48 by Myxobacter Sp. AL-1 has not been directly proven.
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