How Molecular Charge Affects Tumor Permeability
Author Information
Author(s): Dellian M, Yuan F, Trubetskoy V S, Torchilin V P, Jain R K
Primary Institution: Department of Otorhinolaryngology, University of Munich
Hypothesis
Molecular charge influences the microvascular permeability of macromolecules in solid tumors.
Conclusion
Positively charged molecules extravasate faster in solid tumors compared to similar-sized compounds with neutral or negative charges.
Supporting Evidence
- Cationized BSA and IgG had more than two-fold higher permeability than anionized counterparts.
- The study used intravital fluorescence microscopy to quantify fluorescence in tumor tissue.
- Cationic molecules cleared from plasma approximately twice as fast as anionic ones.
Takeaway
This study found that positively charged proteins can move into tumors more easily than negatively charged ones, which could help in delivering drugs.
Methodology
The study measured tumor microvascular permeability using fluorescently labeled proteins in a mouse model.
Potential Biases
Potential bias in the selection of protein modifications and their effects on permeability.
Limitations
The study was conducted in a specific mouse model, which may not fully represent human tumors.
Participant Demographics
Severe combined immunodeficient (SCID) mice were used in the study.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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