Canine Distemper Virus and HeLa Cells
Author Information
Author(s): Del Puerto Helen L, Martins Almir S, Milsted Amy, Souza-Fagundes Elaine M, Braz Gissandra F, Hissa Barbara, Andrade Luciana O, Alves Fabiana, Rajão Daniela S, Leite Rômulo C, Vasconcelos Anilton C
Primary Institution: Universidade Federal de Minas Gerais, UFMG, Brazil
Hypothesis
Canine distemper virus (CDV) can induce apoptosis in HeLa cells derived from cervical cancer.
Conclusion
CDV infection leads to apoptosis in HeLa cells through the intrinsic pathway, activating caspase-3 without involving caspase-8.
Supporting Evidence
- CDV infection resulted in significant DNA fragmentation in HeLa cells.
- Caspase-3 was activated in HeLa cells infected with CDV.
- Caspase-8 expression did not change in CDV infected HeLa cells.
Takeaway
This study found that a virus called canine distemper can make cancer cells die by a special process called apoptosis, which is like a cell's way of saying goodbye.
Methodology
HeLa cells were infected with CDV, and apoptosis was assessed using flow cytometry, real-time PCR, and immunocytochemistry.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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