In vitro and in vivo inhibitory effect of three Cox-2 inhibitors and epithelial-to-mesenchymal transition in human bladder cancer cell lines
2011

Effects of Cox-2 Inhibitors on Bladder Cancer

Sample size: 24 publication 10 minutes Evidence: moderate

Author Information

Author(s): Adhim Z, Matsuoka T, Bito T, Shigemura K, Lee K-M, Kawabata M, Fujisawa M, Nibu K, Shirakawa T

Primary Institution: Kobe University Graduate School of Medicine

Hypothesis

The study investigates the effect of Cox-2 inhibitors on reversing epithelial-to-mesenchymal transition (EMT) and inhibiting tumor growth in bladder cancer cells.

Conclusion

Etodolac at clinical doses exhibited induced in vitro and in vivo anti-tumor effects and reversal effect of EMT in T24 cells.

Supporting Evidence

  • Etodolac showed the highest tumor growth inhibition in T24 cells in vivo.
  • Etodolac reduced SNAIL mRNA and vimentin expression while inducing E-cadherin expression.
  • Cell migration was significantly inhibited by etodolac treatment.

Takeaway

This study found that a drug called etodolac can help stop bladder cancer cells from growing and changing into a more dangerous form.

Methodology

The study used three Cox-2 inhibitors on three human bladder cancer cell lines and a mouse model to assess their effects on tumor growth and EMT.

Limitations

The study primarily focused on one cell line (T24) and may not fully represent the effects on other bladder cancer types.

Participant Demographics

The study involved athymic BALB/c mice and human bladder cancer cell lines.

Statistical Information

P-Value

P<0.0001

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1038/bjc.2011.262

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