Measuring Triglycerides from Dried Blood Spots
Author Information
Author(s): Quraishi Rizwana, Lakshmy Ramakrishnan, Prabhakaran Dorairaj, Mukhopadhyay Ashok Kumar, Jailkhani Bansilal
Primary Institution: All India Institute of Medical Sciences (AIIMS), New Delhi, India
Hypothesis
Can triglycerides be effectively measured from blood dried on filter paper?
Conclusion
The study found that triglycerides can be accurately extracted from dried blood spots, making this method viable for use in developing countries.
Supporting Evidence
- The method showed a high intraclass correlation of 0.96 when comparing dried blood spots to plasma samples.
- Mean recovery from dried blood was 99.6%.
- Triglyceride concentrations remained stable for 30 days at room temperature.
Takeaway
You can measure fat in your blood using a tiny dried blood spot on paper, which is easier to collect and send than regular blood samples.
Methodology
Blood was collected, dried on filter paper, and triglycerides were extracted using methanol and measured with an enzymatic method.
Potential Biases
Potential bias in sample selection as participants were chosen from a single center.
Limitations
The study was limited to a specific population and may not be generalizable to all demographics.
Participant Demographics
Patients visiting a centralized laboratory for lipid investigations.
Statistical Information
P-Value
p<0.000
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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