Understanding Cytokinesis in Fission Yeast
Author Information
Author(s): Saberianfar Reza, Cunningham-Dunlop Stephen, Karagiannis Jim, Polymenis Michael
Primary Institution: Department of Biology, University of Western Ontario
Hypothesis
Does the Ser-2 phosphorylation of the RNA polymerase II CTD affect gene expression related to cytokinesis in fission yeast?
Conclusion
Impairment of Ser-2 phosphorylation leads to selective changes in the expression of a limited set of genes involved in cytokinesis without causing widespread transcriptional defects.
Supporting Evidence
- Mutants impaired in Ser-2 phosphorylation show little change in transcription levels for most genes.
- Only a small subset of genes related to cytokinesis are differentially regulated in response to Ser-2 phosphorylation impairment.
- Genetic analysis indicates that Lsk1p and Aip1p act in a linear pathway for cytokinesis regulation.
Takeaway
This study looks at how a specific protein modification affects cell division in yeast, showing that it changes some genes but not others.
Methodology
The study involved creating mutant strains of fission yeast and analyzing their gene expression profiles using microarray technology.
Potential Biases
Potential bias in interpreting the significance of differentially expressed genes due to the limited scope of the analysis.
Limitations
The study primarily focuses on a limited set of genes and may not capture the full complexity of gene regulation in fission yeast.
Participant Demographics
Fission yeast strains used in the study.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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