Generic Residue Numbering of the GAIN Domain of Adhesion GPCRs
Author Information
Author(s): Florian Seufert, Guillermo Pérez-Hernández, Gáspár Pándy-Szekeres, Ramon Guixà-González, Tobias Langenhan, David E. Gloriam, Peter W. Hildebrand
Primary Institution: Leipzig University, Medical Faculty, Leipzig, Germany
Hypothesis
The study proposes a generic residue numbering scheme for GAIN domains to facilitate information integration across different species and receptors.
Conclusion
The GAIN-GRN scheme enables researchers to better understand and compare GAIN domain functions and mutations across species.
Supporting Evidence
- The GAIN domain is a hotspot for pathological mutations.
- The GAIN-GRN scheme was implemented in the GPCR database (GPCRdb).
- Statistically cancer-enriched positions were identified in human aGPCRs.
- The study provides a robust strategy to allocate structural homologies.
- Future studies can use the GAIN-GRN for hypothesis generation and experimental rationalization.
Takeaway
This study created a new way to label parts of a protein that helps scientists understand how it works and how it can be affected by diseases.
Methodology
The study used structural alignments of over 14,000 modeled GAIN domain structures to create a generic residue numbering scheme.
Limitations
The study may not account for all structural variations in GAIN domains across different species.
Digital Object Identifier (DOI)
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