Selective Decrease of Components of the Creatine Kinase System and ATP Synthase Complex in Chronic Chagas Disease Cardiomyopathy Energy Metabolism Enzymes in CCC Myocardium
2011

Energy Metabolism Impairment in Chronic Chagas Disease Cardiomyopathy

Sample size: 20 publication 10 minutes Evidence: moderate

Author Information

Author(s): Teixeira Priscila Camillo, Santos Ronaldo Honorato Barros, Fiorelli Alfredo Inácio, Bilate Angelina Morand Bianchi, Benvenuti Luiz Alberto, Stolf Noedir Antonio, Kalil Jorge, Cunha-Neto Edecio

Primary Institution: University of São Paulo, São Paulo, Brazil

Hypothesis

The myocardium of patients with chronic Chagas disease cardiomyopathy (CCC) presents an impaired energy metabolism.

Conclusion

CCC myocardium shows significantly reduced levels of protein expression of ATP synthase alpha subunit and total creatine kinase enzyme activity compared to other cardiomyopathy types.

Supporting Evidence

  • CCC myocardium displayed selective reduction of protein levels and activity of enzymes crucial for maintaining cytoplasmic ATP levels.
  • Total CK activity was significantly lower in CCC samples than in IC and IDC samples.
  • Reduced myocardial protein expression of CKM was observed in all cardiomyopathy groups.

Takeaway

Patients with chronic Chagas disease have a problem with energy production in their hearts, which makes it harder for their hearts to work properly.

Methodology

Myocardial samples from CCC, IDC, and IC patients were analyzed for protein and mRNA expression of key enzymes related to ATP production.

Potential Biases

Potential biases in sample selection and analysis methods.

Limitations

The study had a small sample size and may not represent all patients with CCC.

Participant Demographics

Patients included 5 with CCC, 5 with IDC, and 5 with IC, all male.

Statistical Information

P-Value

p<0.01

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pntd.0001205

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