A Simple Device for Making Horizontal Polyacrylamide Gels
Author Information
Author(s): Izzo Vincenzo, Costa Maria A, Di Fiore Renata, Duro Giovanni, Bellavia Daniele, Cascone Eleonora, Colombo Paolo, Gioiale Maria C, Barbieri Rainer
Primary Institution: Istituto di Biomedicina e Immunologia Molecolare, C.N.R.
Hypothesis
Can we create a simple and inexpensive device for preparing horizontal polyacrylamide gels for electrophoresis?
Conclusion
The developed device allows for easy and rapid preparation of horizontal polyacrylamide gels, which can be used for effective electrophoretic fractionation of proteins and DNA.
Supporting Evidence
- The device allows for the preparation of gels as easy and rapid as agarose gel preparation.
- Electrophoretic fractionation of proteins and DNA can be performed using a common horizontal electrophoretic tank.
- The gels can be made in different dimensions and with varying numbers of wells.
Takeaway
Scientists made a new tool that helps them quickly create special gels to separate tiny pieces of proteins and DNA, making their experiments easier.
Methodology
The device uses a Plexiglas block to create horizontal polyacrylamide gels, which are then used for electrophoresis of proteins and DNA.
Digital Object Identifier (DOI)
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