Detection of large molecular weight cytokeratin 8 as carrier protein of CA19–9 in non-small-cell lung cancer cell lines
1999
Detection of a special protein in lung cancer cells
Sample size: 8
publication
Author Information
Author(s): Fujita J, Dobashi N, Ohtsuki Y, Ueda Y, Bandoh S, Yamadori I, Takahara J
Primary Institution: Kagawa Medical University
Hypothesis
Antigenic changes of cytokeratin 8 may occur in some non-small-cell lung cancer cell lines.
Conclusion
A higher molecular weight form of cytokeratin 8 was found in some lung cancer cell lines, which may be involved in cancer spread.
Supporting Evidence
- Cytokeratin 8 is expressed in all non-small-cell lung cancers.
- A higher molecular weight form of CK8 was demonstrated in two of eight NSCLC cell lines.
- This CK8 contained antigenic epitopes of CA19–9.
Takeaway
Scientists looked at a protein in lung cancer cells and found a different version of it in some cells, which might help the cancer grow or spread.
Methodology
Western immunoblot analysis and immunohistological staining were used to analyze cytokeratin 8 in NSCLC cell lines.
Digital Object Identifier (DOI)
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