3D Growth of Colorectal Cancer Cells Enhances Angiogenesis
Author Information
Author(s): María Valcárcel, Beatriz Arteta, Arrate Jaureguibeitia, Aritz Lopategi, Iñigo Martínez, Lorea Mendoza, Francisco J. Muruzabal, Clarisa Salado, Fernando Vidal-Vanaclocha
Primary Institution: Pharmakine Ltd., Bizkaia Technology Park, Derio, Bizkaia-48160, Spain
Hypothesis
The 3D growth of cancer cells increases their angiogenic potential prior to hypoxia.
Conclusion
3D growth of colorectal cancer cells enhances their ability to promote angiogenesis and metastasis.
Supporting Evidence
- Spheroid-derived CT26 cells increased VEGF secretion by 70%.
- Spheroid-derived CT26 cells increased the migration of hepatic sinusoidal endothelium cells by 2-fold.
- CT26 cancer cells significantly increased LFA-1 expression in non-hypoxic avascular micrometastases.
- Angiogenesis increased in both subcutaneous tumors and hepatic metastases produced by spheroid-derived CT26 cells.
Takeaway
When cancer cells grow in 3D shapes, they become better at helping blood vessels grow, which can help them spread in the body.
Methodology
CT26 murine colorectal carcinoma cells were cultured as 3D spheroids and their proangiogenic profile was studied.
Participant Demographics
Syngeneic Balb/c mice (male, 6–8 weeks old) were used for in vivo studies.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website