Quality by Design Approach for the Formulation and Evaluation of Stem Cells Derived Rosmarinic Acid-Loaded Nanofibers as an Anti-Wrinkle Patch: In Vitro and In Vivo Characterizations
2024

Anti-Wrinkle Patch from Plant Stem Cells

Sample size: 30 publication 10 minutes Evidence: high

Author Information

Author(s): Abdelmonem Rehab, Bakr Ahmed, Badawy Ingy, Abd El Maksoud Ahmed Ibrahim, Attia Reem T.

Primary Institution: Misr University for Science and Technology (MUST)

Hypothesis

Can a nanofiber patch loaded with rosmarinic acid from Salvia miltiorrhiza stem cells reduce skin wrinkles?

Conclusion

The study found that the nanofiber patch significantly reduced wrinkles and improved skin quality in a mouse model.

Supporting Evidence

  • Jasmonic acid significantly increased rosmarinic acid production in plant cultures.
  • The nanofiber patch showed a burst release of rosmarinic acid within the first 30 minutes.
  • Histological analysis indicated improved skin structure in treated mice.
  • The medicated patch enhanced antioxidant enzyme levels in the skin.
  • Treatment with the patch reduced lipid peroxidation markers in the skin.

Takeaway

This study shows that a special patch made from plant cells can help make wrinkles less visible and improve skin softness.

Methodology

The study involved culturing plant stem cells, applying elicitors to enhance rosmarinic acid production, and testing the anti-wrinkle effects of a nanofiber patch in mice.

Potential Biases

Potential bias in animal selection and the absence of female subjects may affect the outcomes.

Limitations

The study primarily used male mice, which may limit the generalizability of the results to females.

Participant Demographics

Thirty adult male Swiss Albino mice aged 20-30g.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.3390/pharmaceutics16121598

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