Etoposide Sensitivity and Topoisomerase II Gene Transfer
Author Information
Author(s): Zhou Z, Zwelling L A, Ganapathi R, Kleinerman E S
Primary Institution: The University of Texas MD Anderson Cancer Center
Hypothesis
The study investigates whether adenovirus-mediated transfer of the human topoisomerase II α gene affects sensitivity to etoposide independently of topoisomerase II β levels.
Conclusion
The study concludes that enhanced sensitivity to etoposide is due to increased levels of topoisomerase II α and does not affect topoisomerase II β expression.
Supporting Evidence
- MDA-VP cells were more resistant to etoposide than to amsacrine.
- After gene transfer, sensitivity to etoposide increased significantly in MDA-VP cells.
Takeaway
The researchers found that increasing a specific protein in cancer cells makes them more sensitive to a cancer drug, while another protein's levels stay the same.
Methodology
The study used etoposide-resistant human breast cancer cells and an adenoviral vector to assess changes in protein levels and drug sensitivity.
Digital Object Identifier (DOI)
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