Update on the Anopheles gambiae PEST genome assembly
Author Information
Author(s): Maria V Sharakhova, Martin P Hammond, Neil F Lobo, Jaroslaw Krzywinski, Maria F Unger, Maureen E Hillenmeyer, Robert V Bruggner, Ewan Birney, Frank H Collins
Primary Institution: Center for Global Health and Infectious Diseases, University of Notre Dame
Hypothesis
New approaches can improve the physical map and assembly of the Anopheles gambiae genome.
Conclusion
This study has used new approaches to improve the physical map and assembly of the A. gambiae genome.
Supporting Evidence
- 33% of previously unmapped sequences were placed on the chromosomes.
- 15 unmapped scaffolds were placed in the pericentromeric regions of the chromosomes.
- 1.32 Mbp of sequence was placed in physical gaps between scaffolds.
Takeaway
Scientists worked on the Anopheles gambiae genome to better understand its structure, fixing issues and adding new information about its DNA.
Methodology
The study used in situ hybridization with cDNA and BAC clones to map and orient scaffolds in the genome.
Limitations
The Y chromosome sequence remains highly incomplete and fragmented, and some regions may still contain bacterial contamination.
Digital Object Identifier (DOI)
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