Chicken Immune Responses to In Ovo Stimulation and LPS Challenge
Author Information
Author(s): Slawinska Anna, Dunisławska Aleksandra, Kowalczyk Artur, Łukaszewicz Ewa, Siwek Maria
Primary Institution: Bydgoszcz University of Science and Technology
Hypothesis
Distinct chicken genotypes would respond differently to in ovo stimulation of intestinal microbiota with bioactive compounds followed by immune challenge with LPS.
Conclusion
In ovo stimulation had less of an impact on cytokine gene expression than LPS challenge, with broilers showing stronger inflammatory immune responses than GP native chickens.
Supporting Evidence
- Broiler chickens exhibited stronger innate inflammatory responses than native chickens.
- LPS induced both mucosal and systemic immune responses in broilers but only systemic responses in native chickens.
- In ovo stimulation had less impact on cytokine gene expression than LPS challenge.
Takeaway
This study looked at how different types of chickens react to special treatments before they hatch and how they respond to a germ challenge later. It found that one type of chicken gets more inflamed than the other.
Methodology
Eggs were injected with prebiotics, probiotics, or synbiotics on day 12 of incubation, and chicks were challenged with LPS on day 42 post-hatching; gene expression was analyzed using RT-qPCR.
Limitations
The study does not provide evidence that in ovo stimulation alleviates LPS-induced inflammation in the chicken spleen.
Participant Demographics
Two chicken genotypes: commercial broilers and Green-legged Partridge-like native chickens.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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