A Chinese Herbal Decoction, Danggui Buxue Tang, Stimulates Proliferation, Differentiation and Gene Expression of Cultured Osteosarcoma Cells: Genomic Approach to Reveal Specific Gene Activation
2011

Danggui Buxue Tang and Osteosarcoma Cells

publication Evidence: moderate

Author Information

Author(s): Roy C. Y. Choi, Qiu T. Gao, Anna W. H. Cheung, Judy T. T. Zhu, Faye T. C. Lau, Jun Li, Winnie Z. M. Li, Glanice K. Y. Chu, Ran Duan, Jerry K. H. Cheung, An W. Ding, Kui J. Zhao, Tina T. X. Dong, Karl W. K. Tsim

Primary Institution: The Hong Kong University of Science and Technology

Hypothesis

Does Danggui Buxue Tang stimulate proliferation and differentiation of osteosarcoma cells?

Conclusion

Danggui Buxue Tang significantly enhances the proliferation and differentiation of osteosarcoma cells compared to its individual components.

Supporting Evidence

  • Danggui Buxue Tang increased cell proliferation by approximately 42%.
  • DBT treatment resulted in a 22% increase in alkaline phosphatase activity.
  • 403 genes were specifically regulated by DBT treatment in MG-63 cells.

Takeaway

A Chinese herbal mix called Danggui Buxue Tang helps bone cells grow and develop better than when using just one of its ingredients.

Methodology

The study used cultured MG-63 osteosarcoma cells treated with Danggui Buxue Tang, Radix Astragali, and Radix Angelicae Sinensis, followed by DNA microarray analysis to assess gene expression.

Limitations

The study was conducted in vitro, and the effects in vivo may differ.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1093/ecam/nen085

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