Attenuated Combined Action of Cyclosporine A and Hyperlipidemia on Atherogenesis in Rabbits by Thymoquinone
2011

Thymoquinone Reduces Atherosclerosis in Rabbits Treated with Cyclosporine A and High Cholesterol

Sample size: 30 publication 10 minutes Evidence: moderate

Author Information

Author(s): Ragheb Ahmed, Attia Ahmed, Elbarbry Fawzy, Prasad Kailash, Shoker Ahmed

Primary Institution: University of Saskatchewan

Hypothesis

CsA aggravates hyperlipidemia-induced atherosclerosis by synergistically enhancing the reactive oxygen species (ROS) activity and that TQ ameliorates these effects.

Conclusion

Thymoquinone significantly reduces atherosclerosis and oxidative stress in rabbits treated with cyclosporine A and a high-cholesterol diet.

Supporting Evidence

  • CsA alone did not show a significant effect on serum lipids or induce atherosclerosis.
  • High-cholesterol diet induced atherosclerosis covering 45% of the aorta with plaques.
  • TQ decreased aortic MDA levels by 83% and reduced atherosclerosis by 52% compared to CsA and cholesterol treatment.

Takeaway

This study shows that a natural compound called thymoquinone can help protect rabbits from heart problems caused by a drug and a fatty diet.

Methodology

Thirty female New Zealand White rabbits were divided into five groups and treated with different diets and drugs for 8 weeks, followed by assessments of serum lipids and atherosclerotic changes.

Limitations

The study did not measure oxidized LDL-C levels or tissue CsA levels, and varying CsA dosing was not tested.

Participant Demographics

Female New Zealand White rabbits, aged 6-8 weeks.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1093/ecam/nep225

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