Automation of photochemical kinetic determinations without irradiation of the detection cell
1991
Automation of Photochemical Kinetic Determinations
publication
Evidence: moderate
Author Information
Author(s): M. T. Tena, M. D. Luque de Castro, M. Valcarcel
Primary Institution: University of Córdoba
Hypothesis
Can an open-closed configuration improve continuous kinetic methods based on photochemical reactions?
Conclusion
The developed open-closed system allows for effective kinetic measurements without the need for special flow-cells.
Supporting Evidence
- The method allows for the determination of thioridazine in pharmaceuticals.
- The calibration curve was linear in the range of 0.1-20.0 µg/ml.
- Sampling frequencies achieved were between 24 and 13 h-1.
- The method provides high reproducibility in working conditions.
- Fluorescence intensity increased with longer exposure times.
Takeaway
This study created a new way to measure chemical reactions using light without needing special equipment, making it easier to analyze medicines.
Methodology
The method involved recirculating a sample through a detector and an irradiated coil to measure fluorescence.
Limitations
The method's sensitivity can be affected by the flow rate and exposure time.
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