IFCC Method for Measuring Lactate Dehydrogenase
Author Information
Author(s): Renze Bais, Margaret Philcox
Primary Institution: Institute of Medical and Veterinary Science
Hypothesis
The study aims to optimize the IFCC method for measuring lactate dehydrogenase in serum.
Conclusion
The optimized method provides reliable measurements of lactate dehydrogenase activity in serum, particularly for the heart-specific isoenzyme LD1.
Supporting Evidence
- The method is optimized for the heart-specific isoenzyme LD1, which is important for assessing coronary heart disease.
- Empirical optimization techniques were used to select substrate concentrations and pH.
- The study confirms that the lactate to pyruvate reaction is more linear and suitable for kinetic experiments.
Takeaway
This study helps doctors measure a specific enzyme in the blood that can show how well the heart is doing.
Methodology
The method involves measuring the reversible reaction of lactate to pyruvate using a spectrophotometer at 339 nm, with optimized conditions for pH and substrate concentrations.
Limitations
The method may not provide maximum activity for all isoenzymes of lactate dehydrogenase.
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