Release of a Proinflammatory Lipoprotein by Oral Bacteria
Author Information
Author(s): Karched Maribasappa, Ihalin Riikka, Eneslätt Kjell, Zhong Deyu, Oscarsson Jan, Wai Sun N, Chen Casey, Asikainen Sirkka E
Primary Institution: Umeå University
Hypothesis
Do live A. actinomycetemcomitans cells release the structural outer membrane lipoprotein AaPAL in free-soluble form independent of vesicles?
Conclusion
Free-soluble AaPAL can be extracellularly released in a process independent of vesicles.
Supporting Evidence
- The pal locus and its gene product were confirmed in clinical A. actinomycetemcomitans strains.
- AaPAL was detected from filtrates when strains were incubated in serum or broth.
- Electron microscopy showed that A. actinomycetemcomitans vesicles were larger than the filter pores.
Takeaway
The study found that a specific protein from bacteria can be released into the body without the bacteria breaking apart, which might help the bacteria cause disease.
Methodology
The study used PCR, immunoblotting, and a cell culture insert model to analyze the release of AaPAL from A. actinomycetemcomitans strains.
Limitations
The study did not clarify the exact mechanism of AaPAL release into the extracellular space.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website