Lentivirus Display: Human Antibodies on Cells and Virus Particles
Author Information
Author(s): Taube Ran, Zhu Quan, Xu Chen, Diaz-Griffero Felipe, Sui Jianhua, Kamau Erick, Dwyer Markryan, Aird Daniel, Marasco Wayne A.
Primary Institution: Dana-Farber Cancer Institute, Boston, Massachusetts, United States of America
Hypothesis
Can lentiviral vectors be used for the stable expression of human antibodies on the surface of human cells and virus particles?
Conclusion
The study demonstrates a novel platform for displaying human antibodies on cells and lentiviral particles, which may enhance the discovery of therapeutic antibodies.
Supporting Evidence
- High-level expression of scFvFc antibodies was achieved on human cells.
- Antibody-displaying human cells bound antigens specifically.
- Sulfation of CDR tyrosine residues was demonstrated to enhance binding affinity.
- 106-fold enrichment of antibody-expressing cells was achieved with one round of selection.
- scFvFc displaying human cells could be used in functional biological screens.
Takeaway
This study shows how scientists can use a special virus to help human cells show off antibodies, which can help find new medicines.
Methodology
The study utilized self-inactivating lentiviral vectors to express bivalent scFvFc human antibodies on human cells and lentivirus particles, followed by FACS sorting for selection.
Potential Biases
Potential bias in the selection of antibodies due to the reliance on specific binding assays.
Limitations
The study does not address the long-term stability of antibody expression or the potential for immune responses against the displayed antibodies.
Participant Demographics
Human cells were used for the experiments, but specific demographics are not provided.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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