A Recombinant Avian Infectious Bronchitis Virus Expressing a Heterologous Spike Gene Belonging to the 4/91 Serotype
Author Information
Author(s): Maria Armesto, Sharon Evans, David Cavanagh, Abu-Median Abu-Bakr, Sarah Keep, Paul Britton
Primary Institution: Avian Viral Diseases, Institute for Animal Health
Hypothesis
Can a recombinant avian infectious bronchitis virus (IBV) expressing the spike protein of the pathogenic IBV strain 4/91 provide protection against IBV infections?
Conclusion
The recombinant virus BeauR-4/91(S) conferred protection against homologous challenge with pathogenic IBV 4/91(UK) but offered less cross protection against the heterologous M41-CK strain.
Supporting Evidence
- The recombinant virus BeauR-4/91(S) did not confer pathogenicity to chickens.
- Chickens vaccinated with BeauR-4/91(S) showed lower levels of clinical signs when challenged with 4/91(UK).
- Only one chicken from the vaccinated group showed detectable IBV RNA after challenge.
Takeaway
Researchers created a new vaccine for chickens using a virus that doesn't make them sick, and it worked well against one type of virus but not as well against another.
Methodology
The study involved creating a recombinant IBV and testing its pathogenicity and protective efficacy in chickens through various inoculation and challenge experiments.
Limitations
The study did not include a mock:M41 control group for comparison.
Participant Demographics
8-day-old SPF Rhode Island Red chickens were used in the experiments.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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