Gene Analysis of Lung Cells Exposed to Vanadium Pentoxide
Author Information
Author(s): Ingram Jennifer L, Antao-Menezes Aurita, Turpin Elizabeth A, Wallace Duncan G, Mangum James B, Pluta Linda J, Thomas Russell S, Bonner James C
Primary Institution: The Hamner Institutes for Health Sciences
Hypothesis
What genes are affected in human lung fibroblasts when exposed to vanadium pentoxide?
Conclusion
The study identified various genes involved in inflammation, fibrosis, and repair in response to vanadium pentoxide exposure.
Supporting Evidence
- V2O5 altered more than 1,400 genes in lung fibroblasts.
- Approximately 300 genes were induced while over 1,100 genes were suppressed.
- Induced genes included those involved in inflammatory and immune responses.
- Suppressed genes were associated with growth arrest and cell cycle regulation.
Takeaway
When lung cells are exposed to a chemical called vanadium pentoxide, many genes change their activity, which might lead to lung problems.
Methodology
Human lung fibroblasts were exposed to vanadium pentoxide, and gene expression was measured using microarray analysis and validated by RT-PCR.
Participant Demographics
Normal adult human lung fibroblasts were used in the study.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website