Identifying Imprinted Genes in Neonatal Mouse Brain
Author Information
Author(s): Wang Xu, Sun Qi, McGrath Sean D., Mardis Elaine R., Soloway Paul D., Clark Andrew G.
Primary Institution: Cornell University
Hypothesis
Can we identify novel imprinted genes in the neonatal mouse brain using transcriptome-wide sequencing?
Conclusion
The study identified 26 genes with parent-of-origin dependent differential allelic expression, including three novel imprinted genes.
Supporting Evidence
- Identified 26 genes with differential allelic expression.
- Verified 17 of the candidate genes using Pyrosequencing.
- Discovered three novel imprinted genes in neonatal brain.
Takeaway
Researchers looked at baby mice's brains to find special genes that only work from one parent. They found some new ones that scientists didn't know about before.
Methodology
Illumina/Solexa sequencing of transcriptomes from reciprocal F1 mouse neonatal brains.
Potential Biases
Potential bias due to reliance on specific mouse strains and the limitations of the sequencing technology.
Limitations
The study only covered a limited number of genes and may not represent all imprinted genes in different tissues or developmental stages.
Participant Demographics
Neonatal mice from reciprocal crosses of AKR/J and PWD/PhJ strains.
Statistical Information
P-Value
0.024
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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