TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function
2008

TAF3 Interacts with p53 and Inhibits Its Function

publication Evidence: high

Author Information

Author(s): Bereczki Orsolya, Ujfaludi Zsuzsanna, Pardi Norbert, Nagy Zita, Tora Laszlo, Boros Imre M, Balint Eva

Primary Institution: University of Szeged

Hypothesis

How does TAF3 interact with p53 and affect its transcriptional activity?

Conclusion

TAF3 is identified as a negative regulator of p53 transcription activation function.

Supporting Evidence

  • TAF3 interacts with both Drosophila and human p53.
  • Elevated TAF3 expression inhibits transcription activation by p53 in human cell lines.
  • TAF3 decreases the level of the p53 protein without affecting its mRNA level.

Takeaway

TAF3 is a protein that can stop another protein called p53 from doing its job, which is to help protect cells from damage.

Methodology

Yeast two-hybrid assays and in vitro binding assays were used to study the interactions between TAF3 and p53.

Statistical Information

P-Value

p<0.01

Statistical Significance

p<0.01

Digital Object Identifier (DOI)

10.1186/1471-2199-9-57

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